Modeling & Biochemical Analysis: Master Regulator of Pancreas Development
Help me give my students lodging to attend and present at the ASBMB Conference in San Diego, California
I teach science at all levels from non-native language speakers to those enrolled in AP science courses at an inner-city school in Texas. The majority of my students are Hispanic and will be the first in their family to attend a university after graduation. My passion is to build strong relationships with my students that last for a lifetime. This four-year process begins by first reaching their hearts and second, to hook their minds in a grand journey that teaches them to be empowered, tenacious, and lifelong learners and to become excited about the life that awaits them after their post-secondary degrees. I arrive at school at 6:00 AM each day and by 7:00 AM my students are in my room ready for tutoring, independent biomedical research projects and to build strong relationships with their peers. In education many of my students are classified as "at risk", over the past thirty years in education I would say that they are amazing and full of curiosity, willing extremely able to reach the lofty goals that they have set for themselves.
Biomedical research students study the PTF-L complex by performing structural modeling, over-expression and purification of the heterotrimeric complex composed of Ptf1a, E12 and RBPJ. Our students begin their research project during their freshman year and culminate this grand journey by presenting their research in the open poster session at the largest science meeting in the world, the annual American Society of Biochemistry and Molecular Biology Conference. This unique program allows enthusiastic students to perform hands-on experiments in protein structure, biochemistry, and molecular biology. Students use the National Center for Biological Information website to download the protein sequence for Ptf1a, E12, and RbpJ, perform structural modeling of the PTF1-J complex bound to DNA using Pymol, Jmol perform 3D printing of the PTF1-J complex, over-express the proteins in bacteria, purify the proteins to homogeneity by standard Ni-NATA chromatography and perform biochemical functional assays using native PAGE, western analysis and perform crystallization trials of the PTf1-J complex in order to determine the protein structure of the protein/DNA complex.
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