You're on track to get doubled donations (and unlock a reward for the colleague who referred you). Keep up the great work!
Take credit for your charitable giving! Check out your tax receipts

In a rush to make a major tax-deductible gift before the year's end?
Purchase account credits now, and choose projects later!

In a rush to make a major tax-deductible gift before the year's end? Purchase account credits now, and choose projects later!

For direct assistance with credits or anything else, please call The DonorsChoose Scholar Society at (646) 556-9941.

Time is running out! Make your 2025 tax-deductible gift before midnight on December 31.

To use your $50 gift card credits, find a project to fund and we'll automatically apply your credits at checkout. Find a classroom project
Skip to main content

Help teachers & students in your hometown this season!
Use code HOME at checkout and your donation will be matched up to $100.

Your school email address was successfully verified.

This teacher needs your help!

Dr. Hanono from Chula Vista, CA is requesting books through DonorsChoose, the most trusted classroom funding site for teachers.

See what Dr. Hanono is requesting

Biotechnology in the Classroom: Real World Applications of DNA Science

Help me give my students bacterial transformation kits and DNA analysis kits to learn cutting edge biotechnology techniques and the function of DNA.

FULLY FUNDED! Dr. Hanono's classroom raised $901

This project is fully funded

Celebrating Hispanic & Latinx Heritage Month

Celebrate Hispanic & Latinx Heritage Month

This project is a part of the Hispanic & Latinx Heritage Month celebration because it supports a Latino teacher or a school where the majority of students are Latino.

My Students

Our school is in one of the largest public secondary school districts in the state, and we are one of the largest schools in the district with almost 3,000 students. In recent years, the size of our student body has increased dramatically, and with the growth of our site, the laboratory resources available have not necessarily kept up with the increasing size of the school.

My students are looking for real-world applications of what they learn in high school.

I have students at different high school grade levels in three types of classes. The students in my medical biology and medical accelerated biology classes are mostly in their first year of high school and are energetic and motivated. These are students who chose our school's medical pathway because of their interest in a possible future in medicine. On the other hand, the students in my biotechnology class are upperclassmen with an interest in science and are learning the basic skills to work in a laboratory setting; in fact for many of the biotech students this is their third or fourth year of science.

My Project

Biotechnology is a fast growing field that affects all of our lives more and more each day. As such, students should gain an understanding of some of the basic techniques that are used by researchers all over the world.

Students are more engaged when their work in school can be directly linked to applications in the real world.

The kits purchased though this project will be used in two different types of classes to help students better understand the function of DNA and learn some basic biotech techniques used for human benefits.

My medical biology students will be introduced to genetic engineering through a process called bacterial transformation. Through this process, students will see how a sequence of DNA can be controlled to ultimately code for the trait of an organism. Here, students will introduce a sequence of DNA from a bioluminescent jellyfish that codes for the green fluorescent protein (GFP) into bacteria. Since the generic code is essentially universal, the bacteria will interpret that DNA to make the encoded protein that will affect their trait. In this case, bacteria that take up the jellyfish DNA sequence for GFP should glow green due to the presence of that added DNA. Similar bacterial transformation techniques have been used in the biotech industry for the production of human proteins, like insulin, needed to treat diabetes.

My biotechnology students will examine another aspect of DNA technology. Rather than look at gene expression, they will use separate kits to learn how differences in DNA sequences themselves can be used to distinguish individuals through the analysis of DNA from a simulated crime scene.

Dr. Hanono Eastlake High School Grades 9-12

Equity Focus

This project directly supports students at a historically underfunded school. Learn more about our Equity Focus.

This project will directly impact historically underfunded classrooms.

Chula Vista, CA View local requests

Half of students from low‑income households Data about students' economic need comes from the National Center for Education Statistics, via our partners at MDR Education. Learn more

137 students impacted 5donors

Boost your odds

Add a profile photo in addition to your classroom photo. If you add a photo, it'll show up right here on your project page.

You are more likely to receive funding if you put a face to your name.

Upload a photo

Top rated for efficiency and transparency.

You donate directly to the teacher or project you care about and see where every dollar you give goes.

Expand the "Where your donation goes" section below to see exactly what Dr. Hanono is requesting.

See our finances

TEACHERS, FEELING INSPIRED?

You can start a project with the same resources being requested here!

Where Your Donation Goes

Materials Cost Quantity Total
BACTERIAL TRANSFORMATION KT PGLO BIORAD • Ward's Science $99.09 4 $396.36
FORENSIC DNA FINGERPRINTING KI BIORAD • Ward's Science $134.09 2 $268.18

Materials cost

$664.54

Vendor shipping charges

FREE

Sales tax

$60.81

3rd party payment processing fee

$9.97

Fulfillment labor & materials

$30.00

Total project cost

$765.32

Suggested donation to help DonorsChoose reach more classrooms

$135.06

Total project goal

$900.38

How we calculate what's needed

Total project goal

$900.38

4 Donors

-$292.00

Donations toward project cost

-$269.20

Donations to help DonorsChoose reach more classrooms

-$22.80

match offer

-$583.67

Match offer toward project cost

-$496.12

Match offer to help DonorsChoose reach more classrooms

-$87.55

Excluded support for DonorsChoose

-$24.71

Still needed View calculationHide calculation

$0.00

Our team works hard to negotiate the best pricing and selections available.

View complete list Show less

Give the most requested basic supplies

See teacher requests

Give back to your community

Find opportunities to impact local needs by exploring a map of classroom projects near you.

See local area

Project Activity

{"pmaHash":"","showProjectRecommendations":true,"leadGenEnabled":true,"hideTeacherDetails":false,"schoolState":"CA","teacherPossessivePronoun":"their","PMsManager":null,"schoolGradeType":{"id":4,"name":"High School"},"TeacherPMForms":{},"completingDonationAmount":0,"teacherHasClassroomPhoto":true,"proposalURL":"https://www.donorschoose.org/project/biotechnology-in-the-classroom-real-wor/4340000/","mayHaveReplacements":false,"numStudents":137,"isEquityFocusV1":false,"teacherScreenName":"Dr. Hanono","isEquityFocus":true,"proposalHash":"-1690202486","isApplePayMobileOnly":true,"progressBarPercentage":100.00,"monthlySchoolDonationEnabled":false,"photosPublished":true,"queryString":"","DonorPMForm":null,"shouldTrackEquityFocusV2":true,"schoolCity":"Chula Vista","teachersOnPage":[1535967],"isTeacherViewingTheirOwnProject":false,"minimizedLeadGenEnabled":true,"proposalId":4340000,"proposalMessageFormModel":null,"numActiveProjects":0,"verifyShowPhotos":"","eligibleToBeCopied":false,"schoolId":59388,"schoolName":"Eastlake High School","authorIds":{"1535967":{"owner":"Dr. Hanono","donationSalutations":{}}},"displayedPercentFunded":1.0,"proposalsOnPage":[4340000],"pmaId":"","teacherProfilePhotoURL":"https://cdn.donorschoose.net/images/placeholder-avatars/272/teacher-placeholder-4_272.png?auto=webp","teacherHasProfilePhoto":false,"currentWorkflowStatusCode":21,"teacherChallengeId":259070,"isApplePayEnabled":true,"fundedByTarget":false,"activeProject":null,"donorIdsForImpactPhotoCheck":["NjMyMDgxOA==","NjM3Njg2Ng==","NjY5OTA4NA==","NjY2NTYzMA==","MTUzNTk2Nw=="],"teacherId":1535967,"schoolCounty":"San Diego","fullyFunded":true,"isStudentLed":false,"isExpired":true,"schoolStateObj":{"code":"CA","name":"California"}}