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This teacher needs your help!

Dr. Hanono from Chula Vista, CA is requesting books through DonorsChoose, the most trusted classroom funding site for teachers.

See what Dr. Hanono is requesting

Biotechnology in the Classroom: DNA Differences

My students need bacterial transformation kits and DNA analysis kits to learn cutting edge biotechnology techniques and the function of DNA.

FULLY FUNDED! Dr. Hanono's classroom raised $901

This project is fully funded

Celebrating Hispanic & Latinx Heritage Month

Celebrate Hispanic & Latinx Heritage Month

This project is a part of the Hispanic & Latinx Heritage Month celebration because it supports a Latino teacher or a school where the majority of students are Latino.

My Students

In recent years, the size of our student body has increased dramatically. However, with the growth of our site, the laboratory resources available have not necessarily kept up with the increasing size of the school.

My students are a smart energetic bunch that are eager to learn; they are clearly open to new experiences and like to be challenged.

I have students at different high school grade levels in two types of classes. First, the students in my accelerated biology classes are mostly in their first year of high school and are bright and motivated, their excitement for science is clearly on display and be allowed to flourish. On the other hand, the students in my biotechnology class are upperclassmen with an interest in science and are learning the basic skills to work in a laboratory setting; in fact for many of these students this is their third or fourth year of science.

My Project

Biotechnology is a fast growing field that affects all of our lives more and more each day. As such, students should gain an understanding of some of the basic techniques that are used by researchers all over the world.

To truly understand life one must understand how the information encoded in the DNA of an organism determines the traits of that organism.

Thus to truly understand living organisms, one must understand how the differences in DNA can lead to differences in in individuals. The kits purchased though this project will be used in two different types of classes to help students better understand the function of DNA and learn some basic biotech techniques.

My accelerated biology students will be introduced to genetic engineering through a process called bacterial transformation. Through this process, students will see how a simple chemical sequence in DNA can be controlled to ultimately code for the trait of an organism. In this activity, students will introduce a sequence of DNA (a gene) from a bioluminescent jellyfish that codes for the green fluorescent protein (GFP) into bacteria. And since the generic code on the planet is essentially universal, the bacteria will dully interpret whatever DNA inside them to make the encoded protein that will affect their trait. In this case, bacteria that take up the jellyfish DNA sequence for GFP should glow green due to the presence of that gene, but the glow will not be seen in the bacteria used in the experiment without the introduced DNA.

My biotechnology students will examine another aspect of DNA. Rather than look at gene expression, they will use separate kits to learn how differences in DNA sequences themselves can be used to distinguish individuals through the analysis of DNA from a simulated crime scene.

Dr. Hanono Eastlake High School Grades 9-12

Equity Focus

This project directly supports students at a historically underfunded school. Learn more about our Equity Focus.

This project will directly impact historically underfunded classrooms.

Chula Vista, CA View local requests

Half of students from low‑income households Data about students' economic need comes from the National Center for Education Statistics, via our partners at MDR Education. Learn more

140 students impacted 19donors

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Where Your Donation Goes

Where your donation goes
Materials Cost Quantity Total
BACTERIAL TRANSFORMATION KT PGLO BIORAD • Ward's Science $99.09 4 $396.36
FORENSIC DNA FINGERPRINTING KI BIORAD • Ward's Science $134.09 2 $268.18

Materials cost

$664.54

Vendor shipping charges

 FREE

Sales tax

 $60.81

3rd party payment processing fee

$9.97

Fulfillment labor & materials

$30.00

Total project cost

$765.32

Suggested donation to help DonorsChoose reach more classrooms

$135.06

Total project goal

$900.38

How we calculate what's needed

Total project goal

 $900.38

16 Donors

-$636.33

Donations toward project cost

-$567.13

Donations to help DonorsChoose reach more classrooms

-$69.20

match offer

-$233.17

Match offer toward project cost

-$198.19

Match offer to help DonorsChoose reach more classrooms

-$34.98

Excluded support for DonorsChoose

-$30.88

Still needed View calculationHide calculation

 $0.00

Our team works hard to negotiate the best pricing and selections available.

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As a teacher-founded nonprofit, we're trusted by thousands of teachers and supporters across the country. This classroom request for funding was created by Dr. Hanono and reviewed by the DonorsChoose team.

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