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Dr. Apte from Euless TX is requesting Lab Equipment through DonorsChoose, the #1 classroom funding site for teachers.

Genetically Engineering Yogurt Bacteria to Produce an Antimalarial Peptide

Help me give my students supplies to enable us to clone the cDNA for an antimalarial peptide into yogurt bacteria to make 'Antimalarial yogurt'.

  • $540 goal

Hooray! This project is fully funded

Hooray! This project is fully funded

My Students

My students are good learners. They excel at STEM activities, participate fully when presented with engaging content and teaching and do not hesitate to ask questions about content they do not fully understand. For the most part, they are disciplined, focused, respectful and take responsibility for their education and learning.

The students would be eager to exploit an opportunity to expand the formal school curriculum in the form of a STEM project.

They will present the results of their research project in a peer-reviewed Journal; the Journal of High School Science.

My Project

The Agar, media and plates are to expand and grow the colonies that express the antimalarial peptide. The blood is to innoculate with the malarial parasite, Plasmodium berghei along with the yogurt containing the cDNA of the antimalarial peptide. The extent of hemolysis will be measured by measuring the absorbance of the released hemoglobin using a visible spectrophotometer after centrifugation. We also need the Plasmodium berghei culture that we will order from the American Type Culture Collection and the Plasmid vector from GenScript USA.

Students will learn basic concepts of genetic engineering, electroporation protocols, microbiology and methodology by electroporating the Plasmid containing the cDNA of the antimalarial peptide into the yogurt bacteria using an electroporator.

They will learn the basic concepts of sterility and microbiology by performing autoclaving experiments to sterilize agar plates and aseptically plating the transfected yogurt bacteria on to the plates. Experiments will be performed with blood innoculated with P. berghei and/or the transfected yogurt bacteria. The concepts of scientifically sound experimental design, positive and negative controls, statistical significance and interpretation of data will be emphasized and taught during the project. Students will learn to write a scientific manuscript with quality capable of being published in a peer-reviewed journal. A list of learning objectives follows:

1. Transfection of genetic material using electroporation

2. Centrifugation

3. Steam sterilization

4. Experimental design

5. Statistical methods for data analysis

6. Aseptic manipulation of live micro-organisms and genetic material

7. Culturing, growing and expanding selected bacterial colonies on nutrient media.

8. Ultraviolet-Visible spectroscopy

9. Literature search

10. Cloning cDNA into a suitable plasmid vector

11. Preparation and authoring of a scientific manuscript, its submission to a peer reviewed Journal and publication of a peer-reviewed scientific manuscript.

Dr. Apte
Grades 9-12
More than half of students from low‑income households

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9 donors9 donors have given to this project.

This project will reach 60 students.

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Expand the "Where your donation goes" section below to see exactly what Dr. Apte is requesting.

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Where Your Donation Goes

Materials Cost Quantity Total
Petri Dishes, Polystyrene, Disposable, Sterile, 60 x 15 mm, Case of 500 • Carolina Biological Supply Company $114.61 2 $229.22
Agar Powder, Reagent Grade, 500 g • Carolina Biological Supply Company $64.84 1 $64.84
Bovine Blood, Defibrinated, Pooled, 100 mL • Carolina Biological Supply Company $47.02 1 $47.02
Thumbs-Up Microtubes • Carolina Biological Supply Company $43.65 1 $43.65

Materials cost

$384.73

Vendor shipping charges

$38.47

State sales tax

$0.00

3rd party payment processing fee

$5.77

Fulfillment labor & materials

$30.00

Total project cost

$458.97

Suggested donation to help DonorsChoose reach more classrooms

$80.99

Total project goal

$539.96

How we calculate what's needed

Total project goal

$539.96

8 Donors

-$502.32

Donations toward project cost

-$441.97

Donations to help DonorsChoose reach more classrooms

-$60.35

match offer

-$20.00

Match offer toward project cost

-$17.00

Match offer to help DonorsChoose reach more classrooms

-$3.00

Excluded support for DonorsChoose

-$17.64

Still needed View calculationHide calculation

$0.00

Our team works hard to negotiate the best pricing and selections available.

View complete list Show less

Project Activity

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