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Mr. D. from Wilson NC is requesting Technology through DonorsChoose, the #1 classroom funding site for teachers.

DNA Gel Electrophoresis: \Who\'s DNA is this anyway?\

The cost of SNA gel electrophoresis kit is $385, including shipping and <a target="new" href="http://www.donorschoose.org/html/fulfillment.htm" onclick="g_openWindow('http://www.donorschoose.org/html/fulfillment.htm', 300, 800, 'fulfillwindow');return false;">fulfillment</a>.

  • $408 goal

Hooray! This project is fully funded

Hooray! This project is fully funded

The Daniels Learning Center is a place dedicated to excellence, devotion, and patience toward the future leaders of tomorrow. The “Living Zone,” at the Daniels Learning Center is a unique science classroom that offers students hands-on scientific experiments, practical science principles and concepts relating to their natural surroundings. Science students participate in small group practicum's, individual learning sessions, and tutorial sessions that assist students in understanding the concepts of biology, earth science, or physical science. The objective of the \"DNA Gel Electrophoresis\" project is to introduce high school students to biochemical techniques use in modern genetic laboratories. DNA Gel Electrophoresis is a technique used to separate large macromolecules that differ in size, charge or conformation. Science students will study and perform basic laboratory techniques most widely-used techniques in biochemistry and molecular biology. Electrophoresis involves charged molecules being placed in an electric field, they migrate toward either the positive (anode) or negative (cathode) pole according to their charge. Proteins and nucleic acids are resolved (separated) within a matrix or \"gel\". Most commonly, the gel is cast in the shape of a thin slab, with wells for loading the sample. The gel is immersed within an electrophoresis buffer that provides ions to carry a current and some type of buffer to maintain the pH at a relatively constant value. DNA Electrophoresis techniques used to separate DNA segments use electrical current to move molecules through the gel to the negative pole. The distance the molecules travel relates to their size and shape. Smaller molecules move faster, larger molecules move slower. Students will observe how scientist and health care professionals use Electrophoresis in the real world by: (1) DNA Fingerprinting- Profile of a person's inherited DNA variations (2) Maternity/Paternity Testing- Prove kinship (3) Identification of Disaster Victims (9/11, plane crashes) (4) Forensic Science- CSI (Crime Scene Investigation) (5) Catch a Killer or a Thief Sources of DNA: Skin Fragments, Body Fluids, Hair (with roots attached) The goal of this project offers minority students the opportunity to learn the basic about DNA testing in maternity testing and crime scene investigation.

Mr. D.
Grades 9-12
Nearly all students from low‑income households

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1 donor1 donor has given to this project.

This project will reach 30 students.

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Where Your Donation Goes

Materials Cost Quantity Total
EXPL ELECT OF DYES STUDENT • Carolina Biological Supply Company $161.25 1 $161.25
KOOL-AID COLORS 6 STN KIT • Carolina Biological Supply Company $59.50 1 $59.50
EXPL ELECT OF DYES REFILL • Carolina Biological Supply Company $28.10 1 $28.10
DNA GEL ELECTROPHORESIS POSTER • Carolina Biological Supply Company $15.60 1 $15.60

Materials cost

$264.45

Vendor shipping charges

FREE

State sales tax

3rd party payment processing fee

Fulfillment labor & materials

Total project cost

$334.15

Suggested donation to help DonorsChoose reach more classrooms

$73.35

Total project goal

$407.50

How we calculate what's needed

Total project goal

$407.50

1 Donor

-$384.27

Donations toward project cost

-$335.00

Donations to help DonorsChoose reach more classrooms

-$49.27

Excluded support for DonorsChoose

-$24.27

Still needed View calculationHide calculation

$0.00

Our team works hard to negotiate the best pricing and selections available.

View complete list Show less

Project Activity

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